Spontaneous raman spectra of dipicolinic acid in microcrystalline form

Raman spectra of dipicolinic acid in crystalline and liquid environments.

Raman spectra of dipicolinic acid (DPA) are important for detection of bacterial spores, since DPA and its salts present one of their major components. The implementation of a deeply cooled CCD camera in combination with pulsed excitation at 532 nm allowed measuring well-resolved Raman spectra of the DPA in different forms. Powder preparations, crystals grown from saturated solutions and aqueou...

Levels of Ca2+-dipicolinic acid in individual bacillus spores determined using microfluidic Raman tweezers.

Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many environmental stresses and in spore stability, and Ca-DPA levels in spore populations can vary with spore species/strains, as well as with sporulation conditions. We have measured le...

-dipicolinic acid in individual Bacillus spores determined using microfluidic Raman tweezers

Biosynthesis of dipicolinic acid in Bacillus megaterium.

Very little is known of the precursors of the dipicolinic acid (pyridine-2,6-dicarboxylic acid) in bacterial spores. C'4-2,6-diaminopimelic acid was shown to contribute to the formation of dipicolinic acid in Bacillus cereus var. mycoides (Perry and Foster, 1955; Foster, 1956) but the efficiency of the conversion was low, possibly because of a slow penetration of the cells by the exogenously su...

Two-dimensional multiwavelength fluorescence spectra of dipicolinic acid and calcium dipicolinate.

Dipicolinic acid (DPA) and the Ca2+ complex of DPA (CaDPA) are major chemical components of bacterial spores. With fluorescence being considered for the detection and identification of spores, it is important to understand the optical properties of the major components of the spores. We report in some detail on the room-temperature fluorescence excitation and emission spectra of DPA and its cal...